Quantitative Reverse Transcription-PCR Comparison of Tumor Cell Enrichment Methods

Quantitative reverse transcription-PCR comparison of tumor cell enrichment methods.

platelet plots are not always seen in cryoglobulinemia, although other interferences are usually detected with high efficiency. For comparison, whereas the Technicon H*3 appeared to provide a more immediate visual indication of sample cryoglobulins, large platelet count overestimates were still obtained in most cases. Examination of graphic plots is not used in all laboratories, and its efficie...

Magnetic quantitative reverse transcription PCR: a high-throughput method for mRNA extraction and quantitative reverse transcription PCR.

Over the past few years high-throughput platforms for real-time quantitative PCR have become widely available. The cost of RNA extraction from a large number of samples are, however, quite notable. One method that stands out with respect to free up- or downscaling of sample size and reliability is the isolation of mRNA using oligodeoxythymidylate [oligo(dT)25]-coated magnetic particles. In comb...

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*

Design and optimization of reverse-transcription quantitative PCR experiments.

BACKGROUND Quantitative PCR (qPCR) is a valuable technique for accurately and reliably profiling and quantifying gene expression. Typically, samples obtained from the organism of study have to be processed via several preparative steps before qPCR. METHOD We estimated the errors of sample withdrawal and extraction, reverse transcription (RT), and qPCR that are introduced into measurements of ...

Shedding Assessment of Respiratory Syncytial Virus Transcription-PCR to Viral Culture for Comparison of Quantitative Reverse